Escherichia bacteriophage Qbeta nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC363089 |BNCC

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Escherichia bacteriophage Qbeta nucleic acid reference (Heat inactivated) (Strongly positive)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Growth conditions	37°C;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Application	This reference product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It is an inactivated particle of E. coli phage Qbeta. It can be used for the development of quality control products and standard materials of E. coli phage Qbeta, nucleic acid detection positive control, nucleic acid detection research, etc.
Sharing mode	Public welfare sharing