Listeria monocytogenes DNA lab quality control|BNCC363191 |BNCC

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Listeria monocytogenes DNA lab quality control

Subculture	Basic medium (English name: LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	2
Application	The essential control product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It is a suspension of Listeria monocytogenes inactivated bacteria. It can be used for method establishment, work standard assignment, method confirmation and evaluation, Product quality control, etc.
Sharing mode	Public welfare sharing