Streptococcus pneumoniae DNA lab quality control|BNCC363219 |BNCC

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Streptococcus pneumoniae DNA lab quality control

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl.
Growth conditions	37 ℃,18-24h,5% CO2 + 95% air
Storage conditions	-80 ℃
Safety level	2
Isolation	sputum of 75-year-old male, Phoenix, AZ
Application	The liquid indoor quality control is Streptococcus pneumoniae inactivated bacteria suspension, through fluorescence quantitative pcr combined with digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing