Weiyel Reference Materials
Subculture | BCYE agar (Legionella agar) (English name: BCYE): yeast extract powder 10.0g, activated carbon 2.0g, agar 13.0g 121 ℃,15min |
Subculturing | Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL. |
Growth conditions | 37 ℃;48-72h;5% CO2 |
Storage conditions | -80 ℃ |
Safety level | 2 |
Application | The essential control product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It is an inactivated suspension of Legionella pneumophila. It is obtained by adding stabilizers and digital PCR value and then packaging. It can be used for method establishment and work standard assignment., Method confirmation and evaluation, product quality control, etc. |
Sharing mode | Public welfare sharing |