Enterococcus faecalis DNA lab quality control|BNCC363281 |BNCC

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Enterococcus faecalis DNA lab quality control

Subculture	Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
Morphology	Size: 1-2mm Shape: round Edge: neat Transparency: Opacity Color: white Protuberance: Middle bulge Surface: bright and smooth Texture: moist and viscous
Application	Used for precision testing in related laboratories, it can carry out indoor quality control on the manufacturer of enterococcus faecalis nucleic acid detection reagent, effectively monitor the status of the instrument, personnel operation, and the effectiveness of the kit to ensure the accuracy and reliability of the test results.
Sharing mode	Public welfare sharing