Pseudomonas fluorescens DNA lab quality control|BNCC363297 |BNCC

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Pseudomonas fluorescens DNA lab quality control

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL.
Growth conditions	30°C;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Application	Used for precision testing in related laboratories, it can carry out indoor quality control on the manufacturer of fluorescent Pseudomonas nucleic acid detection reagent, effectively monitor the status of the instrument, personnel operation, and the effectiveness of the kit to ensure the accuracy of the test results And reliability.
Sharing mode	Public welfare sharing