Weiyel Reference Materials
Subculture | Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | 37 ℃;48-72h; Aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | This group B streptococcus DNA standard can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through fluorescence quantitative pcr combined with digital PCR determination. |
Sharing mode | Public welfare sharing |