BK polyomavirus DNA lab quality control|BNCC364860 |BNCC

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BK polyomavirus DNA lab quality control

Subculture	EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 37 ℃; Culture time 14-23 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Morphology	cell degeneration; cell rounding;
Isolation	The strain was isolated in 1970 from the urine of a kidney transplant patient in London.
Application	This reference was developed by Henan engineering center of industrial microbial strain. It is an inactivated particle of BK polyoma virus. It can be used for quality control of BK polyoma virus, development of standard substance, positive control of nucleic acid detection, nucleic acid detection research, etc.
Sharing mode	Public welfare sharing