Weiyel Reference Materials
Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
Growth conditions | 37 ℃,18-24h,5% CO2 + 95% air |
Storage conditions | -80 ℃ |
Safety level | 0 |
Application | This Streptococcus pneumoniae DNA standard can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through fluorescence quantitative pcr combined with digital PCR determination. |
Sharing mode | Public welfare sharing |