Mycobacterium scrofulaceum DNA standard|BNCC363833 |BNCC

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Mycobacterium scrofulaceum DNA standard

Subculture	7H10 agar medium (7H10): 0.5g of ammonium sulfate, 1.5g of potassium dihydrogen phosphate, 1.5g of disodium hydrogen phosphate, 0.4g of sodium citrate, 0.025g of magnesium sulfate, 0.5mg of calcium chloride, 0.001g of zinc sulfate, 0.001g of copper sulfate, 0.5g of sodium L-glutamate, 0.04g of ammonium citrate, 0.001g of pyridoxine hydrochloride, 0.5mg of biotin, 0.25mg of malachite green, 15.0g of agar, 5ml of glycerol and 900ml of distilled water, heat, stir and dissolve, boil for 1 minute with a PH value of 6.4-6.8. High pressure sterilization at 121 ℃ for 10 minutes. When cooling 50-55 ℃, add 100ml of OADC additive for filtration and sterilization, mix well, and pour into a plate or test tube.
Subculturing	(1) Prepare the above 2 plates; (2) After disinfecting the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; (3) Absorb 0.5mL of sterile water into the freeze-drying tube, fully dissolve the bacterial powder, then punch into 2 plates, 200 μL/piece, and coat evenly; (4) Place the flat plate under the above culture conditions for cultivation, and the strain can be used after growing out.
Growth conditions	37 ℃;3-5 days; Aerobic;
Storage conditions	2-8 ℃
Safety level	1
Morphology	Round, irregular edges, opaque, pale yellow on the front, convex in the middle, rough surface, dry texture, G (blue-purple), bacillus, purity: pure
Sharing mode	Public welfare sharing