Mycobacterium marinum DNA standard|BNCC363834 |BNCC

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Mycobacterium marinum DNA standard

Subculture	7H10 agar medium (7H10): 0.5g of ammonium sulfate, 1.5g of potassium dihydrogen phosphate, 1.5g of disodium hydrogen phosphate, 0.4g of sodium citrate, 0.025g of magnesium sulfate, 0.5mg of calcium chloride, 0.001g of zinc sulfate, 0.001g of copper sulfate, 0.5g of sodium L-glutamate, 0.04g of ammonium citrate, 0.001g of pyridoxine hydrochloride, 0.5mg of biotin, 0.25mg of malachite green, 15.0g of agar, 5ml of glycerol and 900ml of distilled water, heat, stir and dissolve, boil for 1 minute with a PH value of 6.4-6.8. High pressure sterilization at 121 ℃ for 10 minutes. When cooling 50-55 ℃, add 100ml of OADC additive for filtration and sterilization, mix well, and pour into a plate or test tube.
Growth conditions	37 ℃;3-5 days; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing