Streptococcus agalactiae DNA standard|BNCC363442 |BNCC

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Streptococcus agalactiae DNA standard

Subculture	Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control product is inactivated particles, which need to be used after nucleic acid extraction, Extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	1
Application	The essential control product is developed by the Henan Industrial Microbial Strain Engineering Technology Research Center. It is an inactivated suspension of Staphylococcus epidermidis. It is obtained by adding a stabilizer and a digital PCR value and then packaging. It can be used for method establishment and work standard assignment., Method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing