Five kinds of diarrhoeal Escherichia coli nucleic acid detection kits (Multiplex fluorescent PCR method)|BNCC361407 |BNCC

Essential Information Related Products

Five kinds of diarrhoeal Escherichia coli nucleic acid detection kits (Multiplex fluorescent PCR method)

Subculturing	1. Experimental preparation: dissolve various components in the kit on ice before use, mix slightly and centrifuge briefly, and then place them on ice for later use; 2. Sample processing: sample preparation according to "GB4789.6-2016 diarrhea Escherichia coli test; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 21 μL, template 4 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 50 ℃ 30s 40cycle;
Storage conditions	-20 ℃
Safety level	1
Application	This test kit is suitable for enteropathogenic Escherichia coli (EPEC), enterohemorrhagic Escherichia coli (EHEC)/Shiga toxin-producing Escherichia coli (STEC), intestinal invasive Escherichia coli (EIEC), enterotoxigenic Escherichia coli (ETEC) and intestinal agglomeration Escherichia coli (EAEC) in samples such as water samples of feces, suspected contaminated water and food. Detection, to determine five types of diarrheal Escherichia coli infection.
Sharing mode	Public welfare sharing