Biochemical ldentification Kit for shigella|BNCC361991 |BNCC

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Biochemical ldentification Kit for shigella

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	3~5 single colonies were selected from the plate and mixed with 5mL sterile PBS to prepare bacterial suspension. Take 100 μL of bacterial suspension and inoculate each trace of vials and test tubes (solid medium directly pick single colony inoculation). The inoculated vials and test tubes are then covered with rubber stoppers, cultured with semi-plug, upright in the groove of the inner support, or placed in a suitable bottle holder, cultured in an incubator at 35-37 ℃ for 24-48h, and the results are interpreted with reference to "GB4789.5-2012 Food Microbiological Examination Shigella Test.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment;
Storage conditions	2-8 ℃
Safety level	1
Application	It is suitable for the physiological and biochemical identification of Shigella in "GB4789.5-2012 Food Microbiological Examination Shigella Examination.
Sharing mode	Public welfare sharing