Staphylococcus aureus nucleic acid detection kit (Multiplex fluorescence PCR method)|BNCC361200 |BNCC

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Staphylococcus aureus nucleic acid detection kit (Multiplex fluorescence PCR method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Experimental preparation: Before the experiment, refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample; The various components in the kit are dissolved on the ice before use, slightly mixed and temporarily centrifuged, and then placed on the ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 51 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channels: FAM, VIC.
Growth conditions	Culture temperature 37 ℃; Culture time 20 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	2
Isolation	Human lesion
Application	Rapid detection of Staphylococcus aureus in food and cosmetics
Sharing mode	Public welfare sharing