Weiyel Reference Materials
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| Subculture | EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS |
| Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
| Growth conditions | Culture temperature 37 ℃; Culture time 2-4 days; Gas environment 5% CO2 + 95% air; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Isolation | Human adenovirus strain 11 was Slobitski isolated from human feces in Massachusetts. |
| Application | The human adenovirus 11 DNA liquid indoor quality control product can be used as real-time fluorescence quantitative PCR, digital PCR, DNA probe hybridization detection and high-throughput sequencing methods, quantitative and qualitative analysis of human adenovirus 11, and can also be used in the field of HAdV typing identification and quality control of detection platform. |
| Sharing mode | Public welfare sharing |
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