Mycobacterium smegmatis DNA standard|BNCC364110 |BNCC

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Mycobacterium smegmatis DNA standard

Subculture	EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS
Subculturing	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃; Culture time 5 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	1
Application	The DNA standard of Mycobacterium smegmatis, by fluorescence quantitative pcr combined with digital PCR value, can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing