Quality control sample of total bacterial count in drinking water|BNCC360334 |BNCC

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Quality control sample of total bacterial count in drinking water

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biological safety cabinet, immediately adding 5mL of sterile water or sterile physiological saline to re-dissolve the freeze-dried quality control product, and fully mixing it to prepare a sample suspension, which is a quality control sample with a concentration of 3000~9000CFU/mL.
Growth conditions	37°C, aerobic, 18-24h;
Storage conditions	-20 ℃, cold storage away from light; Ice bag transportation
Safety level	2
Application	GB/T 5750.12-2006 Standard Test Method for Drinking Water Microbial Indicators Total Colony Count Method "HJ 1000-2018 Determination of Total Bacteria in Water Quality Plate Counting Method" The number of strains required for production is: BNCC269342, BNCC286903, BNCC238481 and BNCC293727.
Sharing mode	Public welfare sharing