Water quality total coliform negative quality control sample (paper rapid method)|BNCC360413 |BNCC

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Water quality total coliform negative quality control sample (paper rapid method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 6 mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biosafety cabinet, 6 mL of sterile water is immediately added, and the vials are shaken after dissolution, and the uniform shaking is the negative quality control sample, with a total concentration of 6 mL and a concentration of 300~3000 CFU/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Water quality total coliform and fecal coliform group paper rapid detection quality control and personnel assessment
Sharing mode	Public welfare sharing