Standard quality control sample of coliform bacteria (MPN counting method)|BNCC360591 |BNCC

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Standard quality control sample of coliform bacteria (MPN counting method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 20min, then opened in the biological safety cabinet, 3-4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is the standard quality control sample with a total concentration of 100mL and a concentration of 10-100MPN/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Quality control and personnel assessment of coliform count in food. Reference Standard "GB4789.3-2016 Food Safety National Standard Food Microbiological Detection Coliform Count" MPN Counting Method.
Sharing mode	Public welfare sharing