Water quality enzyme substrate method quality control sample|BNCC361780 |BNCC

Essential Information Related Products

Water quality enzyme substrate method quality control sample

Subculture	MMO-MUG medium: composition (g/L): ammonium sulfate 5.0; Manganese sulfate 0.0005; Zinc sulfate 0.0005; Magnesium sulfate 0.1; Sodium chloride 10.0; Calcium chloride 0.05; Sodium sulfite 0.04; Amphotericin B 0.001; ONPG 0.5;MUG 0.075;Solanium extract 0.5;HEPES 6.9;HEPES sodium salt; Take 100ml of water sample from a 100ml sterile dilution bottle and add 2.7±0.5g of this product, shake evenly to completely dissolve it, and put it into an incubator at 36±1 ℃ for 24 hours.
Subculturing	Samples require a total of 50L of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 5mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the penlin bottle, and all of them are transferred to the above sterile bottle, which is the standard sample stock solution.
Growth conditions	37 ℃, aerobic; 44.5 ℃, aerobic; Culture time 24 hours;
Storage conditions	-20 ℃, cold storage away from light; Ice bag transportation
Safety level	2
Application	Reference standard: HJ 1001-2018 Enzyme Substrate Method for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality; It is used for the quality control of enzyme substrate method for detection of total coliforms, fecal coliforms and Escherichia coli. The number of strains required for production is BNCC269342, BNCC286903 and BNCC238481.
Sharing mode	Public welfare sharing