Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
Growth conditions | 30 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Morphology | Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white uplift: flat surface: rough texture: dry |
Application | Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product is a heat-inactivated strong positive reference for the evaluation of Bacillus pumilus nucleic acid detection reagent, which is used for early diagnosis and quality control of infection. |
Sharing mode | Public welfare sharing |