Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | This product is a white powder, which is dissolved in sterile water or sterile physiological saline and can be fixed to 250mL. The dissolved liquid is equivalent to 250mL of sewage sample, the concentration range is 10 ~ 1000CFU/mL, and the reference concentration value is 13CFU/mL. |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -20 ℃ |
Safety level | 0 |
Application | "Water Pollutant Discharge Standard for GB18466-2005 Medical Institutions" |
Sharing mode | Public welfare sharing |