Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | This spore suspension is a pure spore suspension, which is obtained by cell separation and collection of spores. It can be used directly, and the corresponding volume can be added according to the test requirements. |
| Growth conditions | 45 ℃;24-48h; Aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| Morphology | The colony diameter is 0.5-1mm, round, with neat edges, opaque, gray-white front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G + (blue-purple), bacilli |
| Application | It is suitable for the production of sterilization effect indicator bacteria and high-density Bacillus coagulans in canned food. |
| Sharing mode | Public welfare sharing |
BNCC362179
Talc powder contaminated by Bacillus atrophicus 9372
BNCC362552
Seawater total bacterial colony count quality control sample
BNCC363185
Fecal coliform positive control quality control sample (Membrane membrane method)
BNCC359576
Water quality fecal coliform positive quality control samples (Paper Rapid Method)
BNCC360408
Bacillus subtilis spore suspension
BNCC358969