Weiyel Reference Materials
Subculturing | Glycerol + NB(1:1) is sterilized at 121 ℃ for 15min, the mixed solution is fully mixed with Escherichia coli phage T1 stock solution at 1:1, injected into 1.5mLEP tubes, injected into each tube at 0.5mL, and stored in a refrigerator at -80 ℃. |
Safety level | 0 |
Application | This E. coli phage T1 quantitative standard quality control, through the cultivation of E. coli host bacteria and phage T1, the collection of phage. The phage titer and lysis efficiency were determined by double-layer agar counting method, which was mainly used for phage PhiX174 positive quality control. |
Sharing mode | Public welfare sharing |