Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method)|BNCC360407 |BNCC

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Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	The sample requires a total of 100 mL of sterile water or sterile normal saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biological safety cabinet, 3-4 mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample with a total concentration of 100 mL and a concentration of 300-15000 MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Water quality total coliform and fecal coliform group paper rapid detection quality control and personnel assessment
Sharing mode	Public welfare sharing