Weiyel Reference Materials
Subculture | Liquid thioglycolate medium (English name: FT): casetone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1±0.2. Sterilization at 121 ℃ for 15min. |
Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
Growth conditions | Culture temperature 37 ℃; Culture time 24-48 hours; Anaerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Application | This product is a Clostridium perfringens nucleic acid reference (heat inactivated) (strong positive), which can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control. |
Sharing mode | Public welfare sharing |