Natioanl standard for Escherichia coli DNA content|BNCC361586 |BNCC

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Natioanl standard for Escherichia coli DNA content

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	1. Take out the standard and balance it to room temperature; 2, fully mixed after a short centrifuge, should try to avoid bubbles.
Growth conditions	37 ℃;18-24 hours; Aerobic
Storage conditions	-20 ℃
Safety level	1
Application	It is suitable for quantitative detection of DNA content of Escherichia coli.
Sharing mode	Public welfare sharing