Weiyel Reference Materials
Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
Growth conditions | Culture temperature 37 ℃; Culture time 3-5 days; Gas environment 5% CO2; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Application | Research on Reagents for Detection of Pathogen Nucleic Acid |
Sharing mode | Public welfare sharing |