Shigella flexneri nucleic acid detection standard|BNCC362380 |BNCC

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Shigella flexneri nucleic acid detection standard

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	This standard should be taken out from the low temperature environment before use and balanced to room temperature. Please centrifuge before opening the lid (12000rmp ,60s) ; After opening the lid, add 100 μL of enzyme-free and sterile water to dissolve, shake evenly, and use after instantaneous centrifugation.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment;
Storage conditions	-20 ℃
Safety level	2
Application	It is suitable for the assessment and verification of laboratory nucleic acid detection capabilities (sensitivity test and specificity test); it can carry out indoor quality control on the relevant laboratories of Shigella flexneri nucleic acid detection, and effectively monitor the status of the instrument, personnel operation, and kit Effectiveness, etc., to ensure the accuracy and reliability of the test results, but not used for reagent evaluation and negative-positive result judgment.
Sharing mode	Public welfare sharing