Clostridium difficile Prevot nucleic acid detection standard|BNCC362388 |BNCC

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Clostridium difficile Prevot nucleic acid detection standard

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	When this standard is used, it is moved to room temperature (20~25 ℃) for re-melting, oscillates evenly, centrifuges briefly, and is temporarily stored for use in a low temperature environment on ice to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃; Culture time 48 hours; Anaerobic gas environment;
Storage conditions	-80 ℃
Safety level	0
Application	It is suitable for the assessment and verification of laboratory nucleic acid detection capabilities (sensitivity test and specificity test); it can carry out indoor quality control on the relevant laboratories of Clostridium difficile nucleic acid detection, effectively monitor the status of the instrument, personnel operation, and the effectiveness of the kit, etc. Ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative-positive result judgment.
Sharing mode	Public welfare sharing