Staphylococcus aureus subsp DNA lab quality control|BNCC363273 |BNCC

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Staphylococcus aureus subsp DNA lab quality control

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
Morphology	The colony diameter is 0.5-1mm, round, with neat edges, opaque, yellow on the front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G + (blue-purple), cocci
Application	The essential control product is a non-fixed value quality control product, which is only used for precision detection in related laboratories. It can be used for the manufacturer of Staphylococcus aureus nucleic acid detection reagent, and the relevant laboratory for Staphylococcus aureus nucleic acid detection. Quality control, effectively monitor the status of the instrument, personnel operation, effectiveness of the kit, etc., to ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative and positive result judgment.
Sharing mode	Public welfare sharing