Weiyel Reference Materials
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| Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculturing | Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL. |
| Growth conditions | Culture temperature 37 ℃; Culture time 48 hours; Gas environment 5% CO2; |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| Morphology | Round, neat edges, opaque, light color, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to pick up, no pigment, G-(red), cocci, purity: pure |
| Application | The essential control product is a non-fixed value quality control product, which is only used for precision detection in related laboratories. It can be used for indoor quality control of the manufacturer of the dried Neisseria nucleic acid detection reagent and the relevant laboratory for the dried Neisseria nucleic acid detection. Effectively monitor the status of the instrument, personnel operation, effectiveness of the kit, etc., to ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative-positive result judgment. |
| Sharing mode | Public welfare sharing |
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