Shigella nucleic acid detection kit (Fluorescent PCR method)|BNCC361199 |BNCC

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Shigella nucleic acid detection kit (Fluorescent PCR method)

Subculturing	Sample processing: sample processing shall be carried out according to GB/T 4789.5-2003 Shigella Detection in Food; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL (when the diluted bacteria solution is used as a template to add 10 μL); Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 52 ℃ 30s 40cycle (when diluted bacteria liquid is used as template for the first step reaction for 3min); Fluorescence channel: FAM;
Storage conditions	-20 ℃; Keep away from light
Safety level	0
Application	It is suitable for the auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing