African swine fever virus nucleic acid detection kit (QPCR)|BNCC361557 |BNCC

Essential Information Related Products

African swine fever virus nucleic acid detection kit (QPCR)

Subculturing	Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15.5 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	It is applied to the specific in vitro amplification of African swine fever virus DNA by fluorescent PCR technology, which cannot be used for clinical diagnosis of diseases.
Sharing mode	Public welfare sharing