Human adenovirus 41 nucleic acid detection kit (QPCR)|BNCC361556 |BNCC

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Human adenovirus 41 nucleic acid detection kit (QPCR)

Subculture	EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS
Subculturing	Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 17 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 60 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 5 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	It is used to use fluorescent PCR technology to carry out specific in vitro amplification research and detection of human adenovirus type 41 DNA, which cannot be used for clinical diagnosis of diseases.
Sharing mode	Public welfare sharing