Klebsiella aerogenes nucleic acid detection kit (QPCR)|BNCC361454 |BNCC

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Klebsiella aerogenes nucleic acid detection kit (QPCR)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Experimental preparation: before the experiment, sample enrichment culture was carried out according to GB 4789.41-2016; All kinds of components in the kit should be dissolved on ice before use, slightly mixed and temporarily centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15.3 μL, template 1 μL, sterile ddH2O8.7μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57.4 ℃, 30s, 40cycle; Fluorescence channel: VIC.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	1
Isolation	Sputum, South Carolina Dept. of Health and Environmental Control
Application	Mainly used to detect the detection of Enterobacter aerogenes in water samples of feces, suspected contaminated water, food and other samples
Sharing mode	Public welfare sharing