Weiyel Reference Materials
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| Subculturing | Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 18 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 55 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescent channel: FAM |
| Storage conditions | -20 ℃, kept away from light |
| Safety level | 1 |
| Application | It is used to use fluorescent PCR technology to carry out specific in vitro amplification research and detection of monkeypox virus DNA, which cannot be used for clinical diagnosis of diseases. |
| Sharing mode | Public welfare sharing |
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Five kinds of diarrhoeal Escherichia coli nucleic acid detection kits (Multiplex fluorescent PCR method)
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