Listeria monocytogenes DNA lab quality control|BNCC370137 |BNCC

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Listeria monocytogenes DNA lab quality control

Subculture	Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃)
Subculturing	① Prepare a test tube and 2 plates containing 5~10mL of liquid culture medium; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL of liquid culture medium into a freeze-drying tube, fully dissolve it, and then return it to the liquid test tube for mixing. ④ Absorb 0.2mL of bacteria into a suspension plate and coat evenly, repeat twice to obtain two plates; ⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.
Growth conditions	37 ℃;18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
Morphology	The colony is 0.5-1mm in diameter, round, opaque, yellow on the front, light in color, convex in the middle, smooth in surface, easy to provoke, G + (blue-purple), bacilli
Sharing mode	Public welfare sharing