Vesicular stomatitis virus RNA lab quality control|BNCC370176 |BNCC

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Vesicular stomatitis virus RNA lab quality control

Subculture	EMEM complete medium (with 2% FBS):EMEM + 2% FBS
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 37 ℃; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Application	The quality control was developed by Henan engineering center of industrial microbial strain, which is a vesicular stomatitis virus inactivated suspension, which can be used for method establishment, work standard assignment, method confirmation and evaluation by adding stabilizer and digital PCR value.
Sharing mode	Public welfare sharing