Bovine herpesvirus 1 DNA lab quality control|BNCC371965 |BNCC

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Bovine herpesvirus 1 DNA lab quality control

Subculture	90%EMEM+10%FBS
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 37 ℃; Culture time 2-7 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Isolation	This strain was isolated from the urine of a male patient with congenital cytomegalovirus inclusion disease.
Application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of bovine herpesvirus 1, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing