Human Coxsackievirus B4 virus RNA lab quality control|BNCC372039 |BNCC

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Human Coxsackievirus B4 virus RNA lab quality control

Subculture	EMEM+2%FBS
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	37 ℃;2-7 days; 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	0
Application	The quality control was developed by Henan engineering center of industrial microbial strain, and was inactivated suspension of human Coxsackie virus B4, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through digital PCR value.
Sharing mode	Public welfare sharing