Weiyel Reference Materials
Subculture | EMEM complete medium (with 2% FBS):EMEM + 2% FBS |
Subculturing | When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing. |
Growth conditions | Culture temperature 37 ℃; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | Coxsackie virus B2 RNA standard, by fluorescence quantitative PCR combined with digital PCR value, can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. |
Sharing mode | Public welfare sharing |