Weiyel Reference Materials
Subculture | EMEM+2%FBS |
Subculturing | When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing. |
Growth conditions | The culture temperature is 37 ℃;1-7 days of culture time; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Application | The standard, which is not infectious and has no biosafety risk, is suitable for qualitative and quantitative determination of human Ecovirus 9 nucleic acid by real-time fluorescence quantitative PCR and other methods, as well as laboratory quality control and kit development and evaluation. |
Sharing mode | Public welfare sharing |