Weiyel Reference Materials
Subculture | Peptone (trypsin hydrolysis):15.0, yeast extract powder: 5.0, glucose: 5.0, sodium thioglycolate: 0.5,L-cystine: 0.5, sodium chloride: 2.5, resazurin: 0.001, agar: 0.75,pH: 7.1±0.2(25 ℃) |
Subculturing | When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing. |
Growth conditions | 37 ℃;3-5 days; anaerobic |
Storage conditions | -80 ℃ |
Safety level | 1 |
Morphology | The colony is 1-2mm in diameter, round, neat edges, opaque, milky white on the front, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G (blue-purple), cocci, purity: pure |
Application | The DNA standard of fuxobacterium nucleatum can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by fluorescence quantitative pcr combined with digital PCR. |
Sharing mode | Public welfare sharing |