Standard quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method)|BNCC360414 |BNCC

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Standard quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is a standard quality control sample with a total concentration of 100mL and a concentration of 100-2000MPN/L.
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Specific use: Quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method) Applicable Standard: GB/T5750.12-2006 "Microbiological Indicators of Standard Test Methods for Drinking Water"
Sharing mode	Public welfare sharing