Reference material of bacillus subtilis spores count|BNCC359087 |BNCC

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Reference material of bacillus subtilis spores count

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	1. According to the arrow sign of Xilin bottle cap, open the strain and dissolved liquid; 2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After being fully dissolved, it can be absorbed in proportion according to the test requirements; 4. Please use up immediately after opening, and do not store it;
Growth conditions	37 ℃, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	Pharmacopoeia test
Sharing mode	Public welfare sharing