Reference material of bacillus atrophaeus spores count|BNCC359096 |BNCC

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Reference material of bacillus atrophaeus spores count

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Before use, take it out from -20 ℃ and balance it at room temperature for 10 minutes, and accurately weigh (1.09±0.01)g(20 ℃) of sterile distilled water to re-dissolve the freeze-dried standard substance with a balance.
Growth conditions	37 ℃, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	Bacillus atrophy spore counting standard material is mainly used for the evaluation of biological safety cabinet personnel, products and cross-contamination protection performance. It can also be used for the evaluation of the collection efficiency of air microbial samplers and planktonic bacteria samplers and the evaluation of disinfection and sterilization effects, which is the reliability of the evaluation results Provide measurement traceability guarantee.
Sharing mode	Public welfare sharing