Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | This spore suspension is a pure spore suspension, which is obtained by cell separation and collection of spores. It can be used directly, and the corresponding volume can be added according to the test requirements. |
Growth conditions | 45 ℃;24-48h; Aerobic |
Storage conditions | 2-8 ℃ |
Safety level | 1 |
Morphology | The colony diameter is 0.5-1mm, round, with neat edges, opaque, gray-white front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G + (blue-purple), bacilli |
Application | It is suitable for the production of sterilization effect indicator bacteria and high-density Bacillus coagulans in canned food. |
Sharing mode | Public welfare sharing |