Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | This spore suspension is a pure spore suspension, which is obtained by cell separation and collection of spores. It can be used directly, and the corresponding volume can be added according to the test requirements. |
| Growth conditions | 45 ℃;24-48h; Aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| Morphology | The colony diameter is 0.5-1mm, round, with neat edges, opaque, gray-white front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G + (blue-purple), bacilli |
| Application | It is suitable for the production of sterilization effect indicator bacteria and high-density Bacillus coagulans in canned food. |
| Sharing mode | Public welfare sharing |
Bacillus subtilis spore suspension
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Fecal coliform standard quality control sample (Membrane membrane method)
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Reference material of total bacterial count in food
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