Escherichia coli bacteriophage T1 standard quality control product|BNCC362950 |BNCC

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Escherichia coli bacteriophage T1 standard quality control product

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Take 5mL of filtered and sterilized Escherichia coli bacteriophage T1 stock solution, add 20mL(1:4) of protective agent, mix thoroughly, put into 7mL of Xilin bottles, put into 1mL of each bottle, insert sterile cotton into the bottle mouth, and put into -80 ℃ refrigerator for preservation.
Growth conditions	Culture temperature 37 ℃;18-24 hours; Aerobic;
Storage conditions	-20 ℃
Safety level	0
Application	This E. coli phage T1 quantitative standard quality control, through the cultivation of E. coli host bacteria and phage T1, the collection of phage. The phage titer and lysis efficiency were determined by double-layer agar counting method, which was mainly used for phage PhiX174 positive quality control.
Sharing mode	Public welfare sharing